AutoNeuron

Automated Neuron Reconstruction

AutoNeuron offers a very significant reduction in the time and effort required to reconstruct neurons in 2D and 3D. An innovative tracing algorithm (patented) quickly explores the entire image volume and identifies neuronal processes and somas. AutoNeuron models neuronal trees as branching structures, complete with branch nodes, roots and endings. Available as a plug-in module for Neurolucida, the reconstruction model created by AutoNeuron can be analyzed, viewed and edited within Neurolucida. Diameters of the axons and dendrites are recorded at each traced point. Somas are reconstructed as a 3D volume using a set of contours.

AutoNeuron can automatically trace
neurons from brightfield, fluorescent
and confocal images.

Reconstruction Capabilities

• Perform automated neuron tracing in minutes on 3D volumes and 2D images
• Full reconstruction of the entire image stack
• Trace 3D and 2D confocal images
• Trace from brightfield and fluorescent images and stacks
• Process width of branching structures is automatically recorded
• Full 3D modeling and morphometric analysis of automatically traced neurons

New in Version 2.0

• Fully Interactive Version – Trace a single process by clicking on any point.
• Full control over seed placement – indicating which processes should be traced.
• Interactive soma reconstruction in 3D and 2D – click on a soma and create a 3D model instantly.
• WorkFlow window guides the user through each step in the process – dramatically simplifying learning.
• Improved controls for sensitivity, curvature, and noise tolerance.
• Noise reduction filters added for image stacks with high noise levels.

Proven Algorithm

AutoNeuron is based on more than ten years of published research in automated tracing of branching structures in biological tissue. MBF is the recognized leader in analysis tools for neuroanatomical research. Hundreds of researchers trust our products for their research and publication needs – we are the proven source for neuroanatomical analysis.

The complexity of the algorithm is hidden
by a carefully designed, easy to use Workflow.
Click to see full image.

Increase Productivity

The product reduces labor by a factor of five to ten compared to what is known as manual tracing – following each neuron process through a stack and clicking along the process. The continual development of this product is being supported by the NIH – it is a critical component for increasing productivity in neuron tracing and reconstruction.

Rapid Reconstruction

The AutoNeuron tracing time for a typical confocal or brightfield stack is under one minute on a standard desktop PC. This saves hours of manual tracing time compared to the current computer assisted approach. In interactive mode, the feedback is instant – view tracing on the fly and make editing corrections immediately if necessary.

Volume Rendering Display

With the optional Solid Modeling Module, you can view the image stack with reconstruction model superimposed in 3D. Rotate the stack and model, zoom in on any area for closer inspection of tracing accuracy. Options are provided to filter the 3D image data and set the transparency level of the traced processes in the model.

Compatible Output

AutoNeuron produces output in the Neurolucida data file format. View and edit reconstructions in Neurolucida. Use Neurolucida Explorer for full morphometric analysis of complex branching structures. Quickly export results to standard document formats for publication.

Autoneuron reconstruction
viewed with the Solid Modeling Module.

Multiple Modalities

AutoNeuron can perform reconstructions from multiple microscope modalities, such as confocal, brightfield and widefield fluorescent images and stacks.

Outstanding Customer Support

MBF is recognized in the neuroscience community as a leader in product support. Our customers have come to appreciate our knowledgeable training and support staff.