MBF Bioscience Announces Launch of MicroDynamix

New Software Application Quantifies Changes in Dendritic Spine Morphology Over Time

Williston, VT — December 10, 2019 — The ability to track the changes that occur in dendritic spine morphology over time is critical to many scientific studies, which is why MBF Bioscience is pleased to announce the launch of MicroDynamix. This powerful new software application helps neuroscientists acquire more information about morphological changes in the brain with impressive speed. MicroDynamix also offers the ability to visualize and quantify dendritic spine morphology over time.

After loading image data acquired at different time points from in vivo and in vitro imaging sessions, MicroDynamix automatically aligns the images in 3D, then reconstructs dendritic branches, detects dendritic spines, and identifies important metrics — such as length, thickness, and overall number, for accurate quantitative comparison.

Since all images are managed within a single framework, the research process is streamlined, saving neuroscientists time in the laboratory that would otherwise be spent locating and manually finding the same spine. MicroDynamix also offers researchers the ability to view two 3D images side-by-side — an invaluable feature for tracking the changes that occur in dendritic spine morphology over time.

Over the course of an experiment, researchers have the ability to upload new images and compare the same region at different time points with MicroDynamix thanks to the software’s sophisticated algorithms. Dendrites and spines are automatically associated across images, so that the same dendrite imaged at any timepoint — two days later, two weeks later, or two months later is automatically detected and identified. The researcher is then able to very clearly view and quantify the changes in morphology that may or may not have occurred.

The software also includes customizable graphs, which give researchers the ability to present their data visually. Key metrics, such as the number and density of spines per time point; head diameter, plane angle, and luminance of individual spines; as well as the total number of spines within a specific region can all be clearly presented in tabular and graph form.

“MicroDynamix provides researchers with the unprecedented capability to get more information about changes in dendritic spines observed in repeated imaging experiments,” says MBF Bioscience President Jack Glaser. “We’re so pleased to announce the launch of this powerful new product for visualizing and quantifying spine morphology over time.”

To learn more about MicroDynamix visit https://www.mbfbioscience.com/microdynamix 

About MBF Bioscience: MBF Bioscience creates quantitative imaging and visualization software for stereology, neuron reconstruction, vascular analysis, c. elegans behavior analysis and medical education, integrated with the world’s leading microscope systems, to empower research.

Our development team and staff scientists are actively engaged with leading bioscience researchers, constantly working to refine our products based on state-of-the-art scientific advances in the field.

Founded as MicroBrightField, Inc. in 1988, we changed our name to MBF Bioscience in 2005 to reflect the expansion of our products and services to new microscopy techniques in all fields of biological research and education. While we continue to specialize in neuroscience research, our products are also used extensively in the research fields of stem cells, lung, kidney, cardiac, cancer, and toxicology.

MBF Bioscience has grown into a global business, with offices in North America, Europe, Japan, and China, and a dealer network active on five continents. Our commitment to innovative products and unrivaled customer support has gained high praise from distinguished scientists who use our products all over the world. Our flagship products Stereo Investigator and Neurolucida are the most widely-used analysis systems for stereology and neuron reconstruction.

For more information visit www.mbfbioscience.com or follow MBF Bioscience on Facebook, Twitter, and LinkedIn, and track our NeuroArt.com contest on Instagram.

 

Better. Faster. More Efficient than Ever Before.

Something big is happening at MBF Bioscience. Something that will make your work better than ever before.

Over the past 32 years we have taken pride in offering our customers the best tools for their research. And we are constantly striving to make things even better for you.

Get ready, because something extraordinary is coming in 2020. Something that will make your work faster and more efficient. Something that can handle big files and analyze them with stunning speed and precision. We are beyond excited to share our big news with you.

Watch this space in 2020 to learn more.

A GPS for the brain and so much more

Scientists use NeuroInfo to help navigate the brain and compare findings across labs

Reproducibility has always been a primary goal in science. But the human effort involved in replicating a research study and analyzing the results, can be considerable. NeuroInfo® is a revolutionary new tool that scientists are using to register whole slide images into a standardized mouse brain atlas in an easy, automated way. Images and subsequent measurements can then be cross-referenced against findings from a myriad of other studies.

NeuroInfo

Coronal mouse brain section from the Laboratory of Systems Neuroscience of Dr. Charles Gerfen, NIMH Bethesda, Maryland

Meeting the demands of users is always a priority for MBF Bioscience, and working with customers like Dr. Charles Gerfen of the National Institute of Mental Health provided a major impetus for developing NeuroInfo into such a revolutionary product.

“The major advance,” said Dr. Gerfen, “is that we’re able to analyze projections from within and between different areas of the cerebral cortex to determine organizational principles of the cerebral cortex,” Along with Dr. Bryan M. Hooks of the University of Pittsburgh School of Medicine, Dr. Gerfen uses NeuroInfo to trace pyramidal neuron projections in Cre-driver mice (Hooks, et al 2018).

As outlined in a study published in Nature Communications, the research team first used MBF Bioscience’s BrainMaker functionality of NeuroInfo to reconstruct four Cre-recombinase driver mouse brains with sections imaged with Neurolucida. They then registered the reconstructed brains into the Allen Mouse Brain Atlas and then collectively visualized experimental data overlain with the atlas to determine exactly how the four populations of cortical pyramidal neurons they were tracking fit within the greater structure of the brain. “Essentially every pixel or image in our original images could be assigned to one of the 2500 brain structures in the Allen Atlas, said Dr. Gerfen.

Continue reading “A GPS for the brain and so much more” »

In Memoriam: Edmund M. Glaser, PhD

Dr. Edmund Glaser devoted his career of more than four decades to the field of neuroscience. Most notably, in 1963, he co-invented computer microscopy, a pioneering method of quantifying the brain’s morphometry. This technology, for the first time, applied computer techniques to the neuroanatomical world, permitting scientists to precisely quantify the brain’s three-dimensional structure. It simplified time-consuming, inexact classical methodologies in an efficient and cost-effective method. By 1995, the year of Dr. Glaser’s retirement, computer microscopy had been adopted by thousands of neuroscience laboratories throughout the world.

Dr.Edmund GlaserDr. Glaser started his college education in his hometown, New York City, studying Electrical Engineering at The Cooper Union. In the midst of his college career, he was drafted and served in the U.S. Army during WWII. His duty took him to Nuremberg, where he was a sound recordist and photographer who documented the Medical War Trials of infamous Nazi physicians. After his military service, he completed his bachelor’s degree in 1949. After doing early project work in communications systems and guided missiles for the U.S. Air Force, he soon became attracted to the emerging fields of computing, information theory, and artificial intelligence. In 1952, he returned to his academic studies in engineering. He received a PhD from Johns Hopkins University in 1960 and then secured a postdoctoral fellowship in its Department of Physiology in the School of Medicine.

In 1963, Dr. Glaser teamed up with Dr. Hendrik Van der Loos, a neuroanatomist at Johns Hopkins, to study the complex morphology of the brain’s cerebral cortex. They encountered the shortcomings of the time’s tedious neuroanatomical techniques and noted the need to revamp the prevailing methods of analyzing neuron morphology and neuronal networks within the cerebral cortex. It was then that they formulated the design and the construction of the first computer microscope.

Computer microscopy at that time was based on the use of analog computer technology. Glaser and Van der Loos demonstrated the great improvements that could made in neuroanatomy by adapting computer technology, it showed the practical way to represent the brain’s structure in its intrinsic three-dimensional reality. In so doing, the quantification of neuroanatomy was wholly revolutionized. Tracing neuronal structures was reduced from hours to minutes and measurement precision was able to achieve fractional micron accuracy. What is more, large assemblies of neuronal networks could be examined in quantitative detail in three dimensions.

Continue reading “In Memoriam: Edmund M. Glaser, PhD” »

Scientists use Vesselucida 360 to quantify brain vasculature in mTBI model

It is not uncommon for war veterans returning home from war-zones like Iraq and Afghanistan to suffer from blast-induced traumatic brain injuries (TBI). In these situations, the most common types of blasts are lower level blasts, the kind that produce mild TBIs (mTBI). Though the effects of a mTBI aren’t visible from the outside, scientists say the blood vessels inside the brain are deeply altered.

In their study of a mouse model of mTBI that mimics the blast exposure associated with human mild TBI, a research team, that includes MBF Bioscience Scientific Director Dr. Susan Tappan, say that low-level blast exposure disrupts the way cells interact with each other within the brain’s neurovascular unit.

Fig:1 Chronic vascular pathology in blast-exposed rats revealed by micro-CT scanning. Two control and two blast-exposed rats were transcardially perfused with the Brite Vu contrast agent at 10 months after blast exposure. Brains were scanned at a resolution of 7.5 μm using equispaced angles of view around 360°, and 3D reconstructions were prepared with Bruker’s CTVox 3D visualization software. a-d MIP images of volume-rendered brain vasculature from two control (a, b) and two blast-exposed (c, d) rats revealed diffuse thinning of the brain vasculature in the blast-exposed rats. Scale bar, 2 mm. e-h Trace sagittal reconstructions used for the automated quantitation from control (e-f) and blastexposed rats (g-h) o-p Higher magnification views of the regions outlined by the boxes in panels (f) and (h). Scale bars, 1 mm for (e-h), and 0.6mm for (o-p). i-n Reconstructions of coronal optical sections from the brains of control (i, k) and blast-exposed (j, l) animals. Panels (i) and (j) correspond approximately to coordinates interaural 12.24–9.48 mm and panels (k) and (l) correspond approximately to coordinates interaural 6.94–3.24 mm. Lateral views of (i) and (j) are shown in (m) and (n), respectively. Vessels were color coded to allow visualization of individual vessels automatically traced by the Vesselucida 360 software. Note the general loss of radial organization in the blast-exposed shown in panel (j). Scale bar, 1 mm for (i-n)

Aiming to mimic an event often experienced by soldiers and military personnel in war-torn regions, the scientists exposed rats to a series of three blasts — one blast per day, over three consecutive days. Though the rats developed behaviors typical to chronic PTSD, their neuronal pathology, at least at the light and electron microscopy levels remained unchanged, according to the study. However, when the researchers examined the rat brains on a vascular level, they found evidence of chronic damage.

Continue reading “Scientists use Vesselucida 360 to quantify brain vasculature in mTBI model” »

Researchers cited MBF Bioscience systems in 24 papers between 1/4/2019 and 1/18/2019

Stereo Investigator:

Branch, A., Monasterio, A., Blair, G., Knierim, J. J., Gallagher, M., & Haberman, R. P. (2019). Aged rats with preserved memory dynamically recruit hippocampal inhibition in a local/global cue mismatch environment. Neurobiology of Aging. doi: https://doi.org/10.1016/j.neurobiolaging.2018.12.015.

Chung, Y., Buechel, B. D., Sunwoo, W., Wagner, J. D., & Delgutte, B. (2019). Neural ITD Sensitivity and Temporal Coding with Cochlear Implants in an Animal Model of Early-Onset Deafness. Journal of the Association for Research in Otolaryngology. doi: 10.1007/s10162-018-00708-w.

Ganeshan, V., Skladnev, N. V., Kim, J. Y., Mitrofanis, J., Stone, J., & Johnstone, D. M. (2019). Pre-conditioning with Remote Photobiomodulation Modulates the Brain Transcriptome and Protects Against MPTP Insult in Mice. Neuroscience, 400, 85-97. doi: https://doi.org/10.1016/j.neuroscience.2018.12.050.

Gao, Ruixuan, Shoh M. Asano, Srigokul Upadhyayula, Igor Pisarev, Daniel E. Milkie, Tsung-Li Liu, Ved Singh, et al. Cortical Column and Whole-Brain Imaging with Molecular Contrast and Nanoscale Resolution. Science 363, no. 6424 (January 18, 2019): eaau8302. doi: https://doi.org/10.1126/science.aau8302. Continue reading “Researchers cited MBF Bioscience systems in 24 papers between 1/4/2019 and 1/18/2019” »

MBF Bioscience research team contributes novel dendritic spine analysis in study published in Science

Combination of new microscopy and expansion tissue preparation methods facilitate better and faster analysis of subcellular neural elements.

Today, the journal Science published a paper authored by a research team led by Dr. Ed Boyden of MIT and Nobel Prize recipient Dr. Eric Betzig of Janelia Research Campus. Among the authors are MBF Bioscience Scientific Director Dr. Susan Tappan and Senior Software Engineer Alfredo Rodriguez. In the paper, the researchers introduce new analyses for neural circuits at nanoscale resolutions.

Combining microscopy methods that create high resolution 3D images from whole brains and tissue that have been made physically larger, the researchers imaged a mouse cortex and fruit fly brain in their study “Cortical column and whole-brain imaging of neural circuits with molecular contrast and nanoscale resolution (Gao et al, 2019).”

By creating enhanced processing and analysis tools in MBF Bioscience’s Stereo Investigator and Neurolucida 360 software, Dr. Tappan and Mr. Rodriguez analyzed these images to obtain comprehensive morphometrics of delicate dendritic spines at a greater accuracy than ever before.

GAO ET AL./SCIENCE 2019

“We combined expansion microscopy and lattice light sheet microscopy (ExLLSM) to image the nanoscale spatial relationships between proteins across the thickness of the mouse cortex or the entire Drosophila brain, including synaptic proteins at dendritic spines, myelination along axons, and presynaptic densities at dopaminergic neurons in every fly neuropil domain.” (Gao et al, 2019)

While several forms of microscopy exist that have the ability to image subcellular neural elements, scientists say that each of these methods is lacking in one way or another. According to the paper, the combination of expansion microscopy with lattice-light sheet microscopy gives the most effective results, while considerably decreasing the time spent carrying out the experiment.

“I believe this type of imaging represents a major milestone in terms of the accuracy that can be achieved in dendritic spine morphometry from light microscopy,” Mr. Rodriguez said.

Continue reading “MBF Bioscience research team contributes novel dendritic spine analysis in study published in Science” »

MBF Bioscience receives NIH funding to support innovative research program on the peripheral nervous system

FOR IMMEDIATE RELEASE

MBF Bioscience Williston, VT – January 9, 2019 – MBF Bioscience is pleased to announce our participation in the Stimulating Peripheral Activity to Relieve Conditions (SPARC) program. Funded by the National Institutes of Health (NIH), this extensive research initiative is a vast collaborative effort, which aims to deepen the understanding of how the peripheral nervous system impacts internal organ function.

“We are honored to be working in collaboration with over 40 research teams in the United States and around the world who are making revolutionary discoveries about how the network of nerves located outside the brain and spinal cord affect organs such as the heart, stomach, and bladder, and what role these nerves play in diseases like hypertension and type II diabetes as well as gastrointestinal and inflammatory disorders,” says Jack Glaser, President of MBF Bioscience.

To facilitate this important research, MBF Bioscience will provide the collaborating research scientists with both software and support. Specifically, we will provide image segmentation tools developed to handle large and diverse amounts of scientific image data. Software applications such as Neurolucida 360®, Tissue Mapper™ and Tissue Maker™ will enable researchers to image and analyze nerves, tissues, and entire organs in 2D and 3D.

“Representing the innervation patterns accurately and robustly is an essential contribution to the generation of representative models that can be used for simulations.  We are working with our partners at the University of Auckland, under the direction of Professor Peter Hunter, to create these models for each organ system that will be an enduring resource for scientists for years to come,” says Susan Tappan, Scientific Director at MBF Bioscience.

Researchers involved in the SPARC program are making important advances in health and medicine, which may lead to the development of new therapies for managing an array of illnesses and disorders. Some examples of research areas include subcutaneous nerve stimulation for arrhythmia control, sensory neuromodulation of the esophagus, and mapping of the neural circuitry of bone marrow. We are thrilled about this opportunity to work in partnership with such an impressive array of research teams on this ground-breaking project.

About MBF Bioscience
MBF Bioscience creates quantitative imaging and visualization software for stereology, neuron reconstruction, vascular analysis, C. elegans behavior analysis, and medical education—integrated with the world’s leading microscope systems—to empower research. Our development team and staff scientists are actively engaged with leading bioscience researchers, and constantly work to refine our products based on state-of-the-art scientific advances.

Founded as MicroBrightField, Inc. in 1988, we changed our name to MBF Bioscience in 2005 to reflect the expansion of our products and services to new microscopy techniques in all fields of biological research and education. While we continue to specialize in neuroscience research, our products are also used extensively in pulmonary, cardiac, kidney, cancer, stem cell, and toxicology research.

Our commitment to innovative products and unrivaled customer support has gained high praise from distinguished scientists all over the world and resulted in MBF expanding into a global business with offices in North America, Europe, Japan, and China. Our flagship products, Stereo Investigator® and Neurolucida®, are the most widely-used analysis systems of their kind.

About SPARC

Stimulating Peripheral Activity to Relieve Conditions (SPARC) is a National Institutes of Health (NIH) program that focuses on understanding peripheral nerves — nerves that connect the brain and spinal cord to the rest of the body — and how their electrical signals control internal organ function. Methods and medical devices that modulate these nerve signals are a potentially powerful way to treat many diseases and conditions, such as hypertension, heart failure, gastrointestinal disorders, type II diabetes, inflammatory disorders, and more.

Researchers cited MBF Bioscience systems in 9 papers between 12/14/2018 and 12/21/2018

Stereo Investigator:

Kumar, A. J., Motta-Teixeira, L. C., Takada, S. H., Lee, V. Y., Machado-Nils, A. V., Xavier, G. F., & Nogueira, M. I. (2018). Behavioral, cognitive and histological changes following neonatal anoxia: male and female rats’ differences at adolescent age. International Journal of Developmental Neuroscience. doi: https://doi.org/10.1016/j.ijdevneu.2018.12.002.

Martínez Cerdeño, V., Hong, T., Amina, S., Lechpammer, M., Ariza, J., Tassone, F., . . . Hagerman, R. (2018). Microglial cell activation and senescence are characteristic of the pathology FXTAS. Movement Disorders, 0(0). doi: 10.1002/mds.27553.

Osipovitch, M., Asenjo Martinez, A., Mariani, J. N., Cornwell, A., Dhaliwal, S., Zou, L., . . . Goldman, S. A. (2018). Human ESC-Derived Chimeric Mouse Models of Huntington’s Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation. Cell Stem Cell. doi: https://doi.org/10.1016/j.stem.2018.11.010.

Neurolucida:

Chaaya, N., Jacques, A., Belmer, A., Richard, D. J., Bartlett, S. E., Battle, A. R., & Johnson, L. R. (2018). Localization of Contextual and Context Removed Auditory Fear Memory within the Basolateral Amygdala Complex. Neuroscience. doi: https://doi.org/10.1016/j.neuroscience.2018.12.004.

Eastwood, B. S., Hooks, B. M., Paletzki, R. F., O’Connor, N. J., Glaser, J. R., & Gerfen, C. R. (2018). Whole Mouse Brain Reconstruction and Registration to a Reference Atlas with Standard Histochemical Processing of Coronal Sections. Journal of Comparative Neurology, 0(ja), e24602. doi: 10.1002/cne.24602.  Continue reading “Researchers cited MBF Bioscience systems in 9 papers between 12/14/2018 and 12/21/2018” »

Researchers cited MBF Bioscience systems in 14 papers between 12/7/2018 and 12/14/2018

Stereo Investigator:

Aldehri, M., Temel, Y., Jahanshahi, A., & Hescham, S. (2018). Fornix deep brain stimulation induces reduction of hippocampal synaptophysin levels. Journal of Chemical Neuroanatomy. doi: https://doi.org/10.1016/j.jchemneu.2018.12.001.

Carrica, L., Li, L., Newville, J., Kenton, J., Gustus, K., Brigman, J., & Cunningham, L. A. (2019). Genetic inactivation of hypoxia inducible factor 1-alpha (HIF-1α) in adult hippocampal progenitors impairs neurogenesis and pattern discrimination learning. Neurobiology of Learning and Memory, 157, 79-85. doi: https://doi.org/10.1016/j.nlm.2018.12.002.

Fowke, T. M., Galinsky, R., Davidson, J. O., Wassink, G., Karunasinghe, R. N., Prasad, J. D., . . . Dean, J. M. (2018). Loss of interneurons and disruption of perineuronal nets in the cerebral cortex following hypoxia-ischaemia in near-term fetal sheep. Scientific Reports, 8(1), 17686. doi: 10.1038/s41598-018-36083-y.

Gibson, E. M., Nagaraja, S., Ocampo, A., Tam, L. T., Wood, L. S., Pallegar, P. N., . . . Monje, M. (2018). Methotrexate Chemotherapy Induces Persistent Tri-glial Dysregulation that Underlies Chemotherapy-Related Cognitive Impairment. Cell. doi: https://doi.org/10.1016/j.cell.2018.10.049. Continue reading “Researchers cited MBF Bioscience systems in 14 papers between 12/7/2018 and 12/14/2018” »